Coding

Part:BBa_K1810002:Design

Designed by:   Group: iGEM15_Stony_Brook   (2015-09-13)


Melittin Optimized for E. coli with Mcherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 947
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 193
    Illegal AgeI site found at 185
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick was created by optimizing the codons for melittin and fusing the sequence with the code for mCherry.

Source

While the amino acid sequence for melittin was derived from Apis mellifica, the genomic sequence was altered by hand for optimization in E. coli. The mCherry sequence was obtained from

References